Effects of extracellular DNA on plasminogen activation and fibrinolysis
AA Komissarov, G Florova, S Idell - Journal of Biological Chemistry, 2011 - ASBMB
The increased levels of extracellular DNA found in a number of disorders involving
dysregulation of the fibrinolytic system may affect interactions between fibrinolytic enzymes
and inhibitors. Double-stranded (ds) DNA and oligonucleotides bind tissue-(tPA) and
urokinase (uPA)-type plasminogen activators, plasmin, and plasminogen with
submicromolar affinity. The binding of enzymes to DNA was detected by EMSA, steady-state,
and stopped-flow fluorimetry. The interaction of dsDNA/oligonucleotides with tPA and uPA …
dysregulation of the fibrinolytic system may affect interactions between fibrinolytic enzymes
and inhibitors. Double-stranded (ds) DNA and oligonucleotides bind tissue-(tPA) and
urokinase (uPA)-type plasminogen activators, plasmin, and plasminogen with
submicromolar affinity. The binding of enzymes to DNA was detected by EMSA, steady-state,
and stopped-flow fluorimetry. The interaction of dsDNA/oligonucleotides with tPA and uPA …
